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Authors: Prof. Robin Williams and Gigi Williams

The Sodium Fluorescein technique:

Although black-and-white film is usually used for retinal fluorescein photography (T-Max 400), the use of the fast colour film is routine for other fluorescein studies such as blood flow to areas of the body, contact lens fittings, corneal abrasions, etc. Ektachrome 400, or P800/1600 for very weak fluorescence, is recommended. In fluorescent antibody techniques, where FITC (fluorescein isothyocyanate) is being used, it is better to use Ektachrome 200 professional film pushed to 400 ISO as this yields a more natural colour rendition of the fluorescence than Ektachrome 400 (which tends to record FITC as yellow rather than apple green). As with all fluorescence photography, expect low levels of emission.

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© 2002 Prof. Robin Williams and Gigi Williams - Disclaimer
Last modified: 3 May 2002