Fluorescence Photography

Authors: Prof. Robin Williams and Gigi Williams

Ultraviolet induced fluorescence:
Practical working method (35mm) - recommendations

Method	Ultraviolet Fuorescence		Control
Film	Ektachrome P800/1600 rated at 3200ISO		Ektachrome 100 or other slide film of choice
Processing	E-6 push processed		E-6 or other
Filter	2B or 2E		None
Lens	105mm Micro Nikkor		105mm Micro Nikkor
Focus Shift	Not necessary		Not necessary
Light Source	Studio flash	SB-140	Studio flash	SB-140
Exposures	f4	f2.8 to f4	Meter as usual	Full Power 1:10 f11 1:8 f11/16 1:4 f16 1:2 f22 1:1 f22

NB. Fluorescence from patient's lesions is always very weak; even with a powerful flashgun (GN 800 in feet at 3200 ISO) expect f4 to f5.6 at 1:8. In the case of very long exposures allowance must also be made for reciprocity failure - which may alter the colour recorded. With some fluorescence there is also a problem of exhaustion extinction - the longer the subject is irradiated the weaker the fluorescence becomes.

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Applications of the ultraviolet technique >

FLUORESCENCE PHOTOGRAPHY

Ultraviolet induced fluorescence: Practical working method (35mm) - recommendations

Ultraviolet induced fluorescence:
Practical working method (35mm) - recommendations