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Authors: Prof. Robin Williams and Gigi Williams

Applications of the Sodium Fluorescein technique:

Application Notes Reference
Brain tumours Described in a series of publications the use of sodium fluorescein for looking at abnormal brain tissue. Concluded that the technique was a valuable aid in determining the presence or absence of abnormal brain tissue in needle biopsy material. Found that metastatic tumours and primary tumours took up fluorescein well. In general claimed that the degree of fluorescence appeared to parallel exactly the degree of malignancy. Moore et al. 1947, 1948, 1950
Brain tumours Described how photographs of the technique were taken that enabled differentiation between arterial and venous vessels and the blood supply to tumours. Feindel, Yamamoto & Hodge 1967
Brain, skin and other tumours Detailed examination of the fluorescein photographic procedures for the brain tumour work described above. Hodge1978


  • Feindel, W., Yamamoto, Y. and Hodge, C., 1967, "Intracarotid fluorescein angiography," Can. Med. J. 96 (1):1-7.
  • Hodge, C., 1978, "Fluorescein angiography of the brain - the photographic procedure," J. Biol. Photogr. 46(2):67- 79.
  • Moore, G., Kohl, D., Marvin, J. and Caudill, C., 1950, .'Biophysical studies of methods utilizing fluorescein and its derivatives to diagnose brain tumours," Radiology 55:344-362.

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Last modified: 3 May 2002