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Authors: Prof. Robin Williams and Gigi Williams

Biomedical Applications: Pathology

Application Notes Reference
Bone and cartilage Showed that bone and cartilage could be differentiated with the ultraviolet technique. Also noted the enhanced surface detail and enhanced colour of fixed specimens. The actual technique used a combination of direct and fluorescence techniques which seemed to help differentiate between the bone and cartilage. Drury and Bullough 1970
Specimens- kidney, jejunal atresia, intestine, lung, heart, brain and secondary neuroblastoma of tibia. Numerous examples of the better definition of specimens using the ultraviolet technique. In the kidney sections it was noted that cystic cavities were more evident in the ultraviolet record. Concluded that ultraviolet absorption is less in compact structures such as bone, cartilage, tendon and .fibrous tissue, and greater in soft tissues such as muscle and fat. In addition blood vessels showed darker in the ultraviolet record when on the surface. Lunnon 1974/76
Cutignola and Bullough 1991
Specimens-intestine, neonatal spine No striking difference from panchromatic photography apart from enhanced surface detail. No advantage for neonatal spine. Marshall 1977


  • Cutignola, A. and Bullough, P., 1991, "Photographic reproduction of anatomic specimens using ultraviolet illumination." Am J Surg Path. 15(11): 1096-1099.
  • Drury, D. and Bullough, P., 1970, "Improved photographic reproduction of bone and cartilage specimens using ultraviolet illumination," Med. Biol. Illus. 20:57-58.
  • Lunnon, R., 1974, "Reflective ultraviolet photography in medicine," MSc Thesis. Faculty of Medicine. University of London.
  • Lunnon, R., 1976, "Reflected ultraviolet photography of human tissues," Med. Biol. Illustr.26:139-144,
  • Marshall, R., 1977, " A study of the selective absorption of ultra-violet and infra-red radiation by some pigmented lesions of the skin," PhD Thesis. CNAA. London.

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Last modified: 3 May 2002